Subject: Breast cancer; HER – 2; Fluorescence in situ hybridisation; Immunohistochemistry
Year: 2018
Type: Article
Title: Correlation of Immunohistochemistry and Fluorescence in Situ Hybridization for HER-2 Assessment in Breast Cancer Patients: Single Centre Experience
Author: Bogdanovska-Todorovska, Magdalena
Author: Kostadinova-Kunovska, Slavica
Author: Jovanovik, Rubens
Author: Krsteska, Blagica
Author: Kondov, Goran
Author: Kondov, Borislav
Author: Petrushevska, Gordana
Abstract: BACKGROUND: Accurate assessment of HER-2 is imperative in selecting patients for targeted therapy. Most commonly used test methods for HER-2 are immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH). We evaluated the concordance between FISH and IHC for HER-2 in breast cancer samples using Food and Drug Administration approved tests. MATERIAL AND METHODS: Archived paraffin tissue blocks from 73 breast cancer patients were used. HER-2 immunostaining was performed using Ventana anti–HER-2 monoclonal antibody. The FISH assay was performed using PathVysion™ HER-2 DNA Probe Kit. RESULTS: Of the 73 cases 68.5% were IHC 0/1+, 15.07% were IHC 2+ and 16.44% were IHC 3+. Successful hybridisation was achieved in 72 cases. HER-2 FISH amplification was determined in 16.67% cases. Ten IHC 3+ and two IHC 2+ cases were FISH positive. Two of the IHC 3+ cases were FISH negative. Concordance rate was 100%, 18.18% and 83.33% for IHC 0/1+, 2+ and 3+ group, respectively. Total concordance was 84.72%, kappa 0.598 (p < 0.0001). The sensitivity of IHC in detecting IHC 2+ and IHC 3+ cases was 16.7% and 83.3%, and the specificity was 85% and 96.67%, respectively. CONCLUSION: The consistency between the methods was highest for IHC negative and lowest for IHC equivocal cases. The immunohistochemistry showed high sensitivity for IHC 2+/3+ cases and high specificity for IHC 3+ cases. Our results support the view that false-positive rather than false-negative IHC results are a problem with HER-2/IHC testing, and that IHC should be used as an initial screening test, but IHC 2+/ 3+ results should be confirmed by FISH.
Publisher: ID Design 2012/DOOEL Skopje
Relation: Open access Macedonian journal of medical sciences
Identifier: oai:repository.ukim.mk:20.500.12188/7938
Identifier: 1857-9655
Identifier: http://hdl.handle.net/20.500.12188/7938Identifier: 10.3889/oamjms.2018.124
Identifier: https://www.id-press.eu/mjms/article/download/oamjms.2018.124/1999Identifier: https://www.id-press.eu/mjms/article/download/oamjms.2018.124/2029Identifier: 6
Identifier: 4
