Subject: β-lactoglobulin; genetic polymorphism; Pramenka breed.
Year: 2018
Type: Article
Title: Polymorphism of β-lactoglobulin in pramenka breed sheep in Bosnia and Herzegovina
Author: Rustempašić, A.
Author: Dokso, A.
Author: Zečević, E.
Author: Hodžić, A.
Author: Hrković-Porobija, A.
Author: Sarić, Z.
Author: Miskoska–Milevska, E.
Author: Popovski, Z.
Author: Brka, M.
Abstract: The aim of this study was to determinate allele frequency β-lactoglobulin in Pramenka breed sheep in Bosnia and Herzegovina. The DNA was extracted from blood of 283 sheep, collected from four flocks around Sarajevo, Bosnia and Herzegovina. The gene β-lactoglobulin was amplified using polymerase chain reaction – restriction fragment length polymorphism (PCR–RFLP) technique. Two genetic variants (A and B) and three genotypes (AA, AB and BB) of β- lactoglobulin have been identified. Genotype frequencies of β-lactoglobulin were with the following values: AA (0.325), AB (0.445), and BB (0.229), while the frequencies of allele A and B were 0.547 and 0.453 respectively. The highest frequency of genotype AA is present in the location Treskavica (38%), genotype AB in the location Vlašić (52%), while the genotype BB is mostly present in location Bjelašnica (29%). The population of Pramenka breed sheep was in Hardy- Weinberg equilibrium. It is expected that in a future the programs in sheep breeding in Bosnia and Herzegovina certainly would take into account the genetic variants of β-lactoglobulin that could contribute to better production and economic benefit.
Publisher: Pakistan Agricultural Scientists Forum
Relation: The Journal of Animal and Plant Sciences
Identifier: oai:repository.ukim.mk:20.500.12188/4942
Identifier: 1018-7081
Identifier: http://hdl.handle.net/20.500.12188/4942Identifier: 28
Identifier: 1
Identifier: 337
Identifier: 340
Identifier: 0.529
