Subject: IL-1β
Subject: wound age
Subject: real-time PCR
Subject: autopsy cases
Subject: human dermal injuries
Year: 2023
Type: Article
Title: TIME-DEPENDENT EXPRESSION OF IL-1β mRNA - A PRELIMINARY STUDY FOR POSSIBLE WOUND AGE MARKER
Author: Bitoljanu, Natasha
Author: Ivcheva, Ana
Author: Belakaposka Srpanova, Viktorija
Author: CHakar, LJupcho
Author: Siamkouri, Rosica
Author: Stankov, Aleksandar
Author: Pavlovski, Goran
Author: Risteski, Sasho
Author: Jakjovski, Zlatko
Abstract: Accurate estimation of wound age is a very important task in forensic medicine field, because it can helps in reconstruction of crime scene. IL-1β is proinflamatory cytokine and plays a major role in wound healing process. The aim of the study is to develop a method for the reliable estimation of dermal injury age. In this study the expression of IL-1β mRNA was evaluated by real-time PCR at skin human wounds with different post-injuried period. We collected 27 human skin wound samples from forensic autopsy cases at our Institute. In our study were include samples from lacerations, incised wounds and stab wounds, with a known time of injury and death. Wound samples were divided into five groups: control group (n=5); first group consisted of cases with immediate death (n=7); second group consisted of cases with survival from 1 hour to 6 hours (n=5); the third group comprised cases with survival from 6 hours to 72 hours (n=3); the fourth group comprised cases with survival from 72 hours to 168 hours (n=7). In this study we detected increased expression levels of IL-1 β mRNA in the second group with survival time from 1 to 6 hours.
Publisher: Macedonian Association of Anatomists and Morphologists
Relation: JMS - Journal of Morphological Sciences
Identifier: oai:repository.ukim.mk:20.500.12188/29277
Identifier: http://hdl.handle.net/20.500.12188/29277Identifier: 10.55302/jms2361121b
