Year: 2022
Type: Article
Title: Development and Optimization of Indirect ELISAs for the Detection of Anti-Capripoxvirus Antibodies in Cattle, Sheep, and Goat Sera
Author: Berguido, Francisco J
Author: Gelaye, Esayas
Author: Liu, Yang
Author: Davaasuren, Batdorj
Author: Krstevski, Kiril
Author: Djadjovski, Igor
Author: Ivanova, Emiliya
Author: Goujgoulova, Gabriela
Author: Loitsch, Angelika
Author: Tuppurainen, Eeva
Author: Chibssa, Tesfaye Rufael
Author: Caufour, Philippe
Author: Samojlović, Milena
Author: Lazić, Sava
Author: Petrović, Tamaš
Author: Vidanović, Dejan
Author: Bertagnoli, Stéphane
Author: Grabherr, Reingard
Author: Diallo, Adama
Author: Cattoli, Giovanni
Author: Lamien, Charles Euloge
Abstract: Sheeppox (SPP), goatpox (GTP), and lumpy skin disease (LSD) are economically significant pox diseases of ruminants, caused by sheeppox virus (SPPV), goatpox virus (GTPV), and lumpy skin disease virus (LSDV), respectively. SPPV and GTPV can infect both sheep and goats, while LSDV mainly affects cattle. The recent emergence of LSD in Asia and Europe and the repeated incursions of SPP in Greece, Bulgaria, and Russia highlight how these diseases can spread outside their endemic regions, stressing the urgent need to develop high-throughput serological surveillance tools. We expressed and tested two recombinant truncated proteins, the capripoxvirus homologs of the vaccinia virus C-type lectin-like protein A34 and the EEV glycoprotein A36, as antigens for an indirect ELISA (iELISA) to detect anti-capripoxvirus antibodies. Since A34 outperformed A36 by showing no cross-reactivity to anti-parapoxvirus antibodies, we optimized an A34 iELISA using two different working conditions, one for LSD in cattle and one for SPP/GTP in sheep and goats. Both displayed sound sensitivities and specificities: 98.81% and 98.72%, respectively, for the LSD iELISA, and 97.68% and 95.35%, respectively, for the SPP/GTP iELISA, and did not cross-react with anti-parapoxvirus antibodies of cattle, sheep, and goats. These assays could facilitate the implementation of capripox control programs through serosurveillance and the screening of animals for trade.
Publisher: MDPI AG
Relation: Microorganisms
Identifier: oai:repository.ukim.mk:20.500.12188/26751
Identifier: 2076-2607
Identifier: http://hdl.handle.net/20.500.12188/26751Identifier: 10.3390/microorganisms10101956
Identifier: https://www.mdpi.com/2076-2607/10/10/1956/pdfIdentifier: 10
Identifier: 10
Identifier: 1956
