Year: 2022
Type: Article
Title: HPV E6/E7mRNA association with interleukin 10 (rs1800872) polymorphism in a group of Macedonian women
Author: Duvlis, Sotirija
Author: Dabeski, Drage
Author: Hiljadnikova Bajro, Marija
Author: Osmani, Dugagjin
Author: Memeti, Shaban
Abstract: Interleukin 10 (IL-10) is an immunosuppressive cytokine and its genetic variants could have an indirect impact on viral biology and human papillomavirus (HPV) E6/E7 messenger RNA (mRNA) expression as well. This study evaluates the association between IL-10-592 C/A (rs1800872) single-nucleotide polymorphism and HPV E6/E7 mRNA expression in a group of women from the Republic of North Macedonia. Using a commercial test, 272 women's cervical samples were analyzed for HPV E6/E7 mRNA and HPV DNA presence. The cases were stratified into three groups: double-positive (n = 108, positive for both tests), negative (n = 51, negative for HPV E6/E7 mRNA and HPV DNA positive), and the control group (n = 113, negative for both tests). The IL-10-592 C/A polymorphism was analyzed using polymerase chain reaction-restriction fragment length polymorphism. The results showed the CC genotype and the C allele frequencies of IL-10-592C/A were significantly higher in double-positive (59.3% and 78.2%) compared to negative group (39.2% and 65.7%), (p = 0.018, confidence interval [CI] = 2.25; 1.14-4.45 and p = 0.016, CI = 1.88; 1.11-3.16, respectively). The CC genotype and C allele of rs1800872 polymorphism were shown to be associated with HPV E6/E7 mRNA but not with HPV DNA positivity, which implies a possible role of this polymorphism in the course of the infection only after HPV onset, and lack of association with the susceptibility to HPV.
Publisher: Wiley
Relation: Journal of Medical Virology
Identifier: oai:repository.ukim.mk:20.500.12188/25595
Identifier: http://hdl.handle.net/20.500.12188/25595Identifier: 10.1002/jmv.27858
Identifier: https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmv.27858Identifier: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jmv.27858Identifier: https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmv.27858Identifier: 94
Identifier: 9
Identifier: 4485
Identifier: 4489
