Subject: idiopathic throbocytopenic purpura (ITP)
Subject: immunoglobulin gene rearrangement
Subject: anti-platelet autoantibodies
Year: 2021
Type: Article
Title: LOW FREQUENCY OF CLONAL B CELL EXPANSIONS IN PATIENTS WITH IDIOPATHIC THROMBOCYTOPENIC PURPURA
Author: Pavkovic, M
Author: Efremov D
Abstract: Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder characterized by an increased platelet destruction caused by autoantibodies directed against platelet membrane glycoproteins (GP), most commonly against GPIb/IX, GPIIb/IIIa and GPIa/IIa. In a few recent studies it has been reported that these antibodies frequently have a restricted light chain phenotype, supporting a clonal origin. In this study we wanted to explore the hypothesis of clonal B cell expansions in chronic ITP. We investigated 40 patients (28 women and 12 men) with chronic ITP for clonal B cell expansions using sensitive RT-PCR technique for analysing Ig-gene rearrangements. RNA was isolated from peripheral blood mononuclear cells separated on a Ficoll gradient. The RNA was converted into cDNA and then amplified using FW3 and IgM or IgG specific oligonucleotides to investigate the clonality of B-cells expressing the respective Ig isotype. The PCR fragments were analyzed on sequencing polyacrylamide gels or with an ABI prism 310 DNA analyser. We detected a monoclonal B cell population in only 1 patient and polyclonal rearrangement with one prominent band in 3 patients in the analysis of IgG heavy chain mRNA. The pattern of IgM heavy chain gene rearrangements was polyclonal in all cases. Our study indicates that clonal B-cell expansions are rare in patients with ITP. Most probably, the clonal B-cell expansion responsible for the production of autoantibodies in ITP, if present, is below the detection limit.
Publisher: Македонско лекарско друштво = Macedonian Medical Association
Relation: Македонски медицински преглед = Macedonian Medical Review
Identifier: oai:repository.ukim.mk:20.500.12188/23764
Identifier: http://hdl.handle.net/20.500.12188/23764
