Subject: veterinary drug residues, mycotoxins, pesticide residues, liquid chromatography electrospray ionisation–tandem mass spectrometry, validation.
Year: 2022
Type: Article
Title: Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry
Author: Hajrulai-Musliu, Zehra
Author: Uzunov, Risto
Author: Jovanov, Stefan
Author: Kerluku, Maksud
Author: Jankuloski, Dean
Author: Stojkovski, Velimir
Author: Pendovski, Lazo
Author: Sasanya, James Jacob
Abstract: Abstract Introduction Multi-class and multi-residue analyses are very complex procedures because of the physico-chemical properties of veterinary drug residues and other contaminants. The purpose of the study was to develop an analytical method for the sensitive determination of 69 analytes in bovine milk by liquid chromatography electrospray ionisation–tandem mass spectrometry. Material and Methods Antimicrobial, anabolic hormone, lactone, β-agonist, mycotoxin and pesticide residues were analysed in 120 raw milk samples from different dairy farms in North Macedonia. Stable isotopically labelled internal standards were used to facilitate effective quantification of the analytes. Results The linear regression coefficients were higher than 0.99, the limits of detection ranged from 0.0036 to 47.94 μg/L, and the limits of quantification ranged from 0.053 to 59.43 μg/L. The decision limit values ranged from 0.062 to 211.32 μg/L and the detection capability from 0.080 to 233.71 μg/L. Average recoveries of the analytes spiked in raw milk were in the range of 70.83% to 109%, intra-day coefficient of variation (CV) values from 2.41% to 22.29%, and inter-day CV values from 3.48% to 23.91%. The method was successfully applied in the testing of bovine milk samples. In five samples residues were detected. They were sulfadimethoxine (in two samples), enrofloxacin, tetracycline and oxytetracycline and were at concentrations below the EU maximum residue limit. Conclusion The method is useful for routine testing for this group of chemical hazards in bovine milk.
Publisher: Walter de Gruyter GmbH
Relation: Integrated Radiometric and Complementary Techniques for Mixed Contaminants and Residues in Food IAEA project D52041
Identifier: oai:repository.ukim.mk:20.500.12188/21569
Identifier: http://hdl.handle.net/20.500.12188/21569Identifier: 10.2478/jvetres-2022-0027
Identifier: https://www.sciendo.com/pdf/10.2478/jvetres-2022-0027Identifier: 66
Identifier: 2
