Subject: mycotoxins; multi-toxin IAC; HPLC-FLD;validation
Year: 2022
Type: Article
Title: Application of reliable cost-effective strategy for analysis of mycotoxins in corn-based foods with HPLC-FLD after multi-toxin immunoaffinity clean-up
Author: Stojanovska Dimzoska, Biljana
Author: Hajrulai Musliu, Zehra
Author: Uzunov, Risto
Author: Angeleska, Aleksandra
Author: Blagoevska, Katerina
Author: Crceva Nikolovska, Radmila
Author: Ilievska, Gordana
Author: Dimitrieska Stojkovic, Elizabeta
Abstract: A different, reliable, and cost-effective strategy for the analysis of aflatoxins, ochratoxin A, and zearalenone in corn-based foods was proposed, including one multi-toxin immunoaffinity column (IAC) sample preparation and three different high-performance liquid chromatography fluorescence detection methods. The analytical procedures were tested and verified, keeping in mind their occurrence at trace levels in corn-based foods. With the validation of the proposed multi-toxin IAC methodology and comparison of the performance characteristics with methods using a single-toxin IAC, we confirmed the reliability of the multi-toxin IAC procedure versus the single-toxin IAC. The methods were validated by revealing satisfactory performance characteristics; for example, the obtained values of limit of detection were significantly lower than the maximum limits for all mycotoxins of concern. In addition, the recovery values were between 70.9 % and 106.1 % for all mycotoxins of interest, with precision values lower than 10.5 %.
Publisher: Society of Chemists and Technologists of Macedonia
Relation: Macedonian Journal of Chemistry and Chemical Engineering
Identifier: oai:repository.ukim.mk:20.500.12188/21568
Identifier: http://hdl.handle.net/20.500.12188/21568Identifier: 10.20450/mjcce.2022.2422
Identifier: https://mjcce.org.mk/index.php/MJCCE/article/download/2422/1055Identifier: https://mjcce.org.mk/index.php/MJCCE/article/download/2422/1055Identifier: 41
Identifier: 1
