Year: 2022
Type: Article
Title: Design and evaluation of nanostructured lipid carriers loaded with Salvia officinalis extract for Alzheimer's disease treatment
Author: Markova, Elena
Author: Taneska, Lea
Author: Kostovska, Monika
Author: Shalabalija, Dushko
Author: Mihailova, Ljubica
Author: Glavas Dodov, Marija
Author: Makreski, Petre
Author: Geskovski, Nikola
Author: Petrushevska, Marija
Author: Taravari, Arben
Author: Simonoska crcarevska, Maja
Abstract: Considering the potential of Salvia officinalis in prevention and treatment of Alzheimer's disease (AD), as well as the ability of nanostructured lipid carriers (NLC) to successfully deliver drug molecules across blood-brain barrier (BBB), the objective of this study was design, development, optimization and characterization of freeze-dried salvia officinalis extract (FSE) loaded NLC intended for intranasal administration. NLC were prepared by solvent evaporation method and the optimization was carried out using central composite design (CCD) of experiments. Further, the optimized formulation (NLCo) was coated either with chitosan (NLCc) or poloxamer (NLCp). Surface characterization of the particles demonstrated a spherical shape with smooth exterior. Particle size of optimal formulations after 0.45 μm pore size filtration ranged from 127 ± 0.68 nm to 140 ± 0.74 nm. The zeta potential was -25.6 ± 0.404 mV; 22.4 ± 1.106 mV and - 6.74 ± 0.609 mV for NLCo, NLCc, and NLCp, respectively. Differential scanning calorimetry (DSC) confirmed the formation of NLC whereas Fourier-transform infrared spectroscopy confirmed the FSE encapsulation into particles. All formulations showcased relatively high drug loading (>86.74 mcg FSE/mg solid lipid) and were characterized by prolonged and controlled release that followed Peppas-Sahlin in vitro release kinetic model. Protein adsorption studies revealed the lowest adsorption of the proteins onto NLCp (43.53 ± 0.07%) and highest protein adsorption onto NLCc (55.97 ± 0.75%) surface. The modified ORAC assay demonstrated higher antioxidative activity for NLCo (95.31 ± 1.86%) and NLCc (97.76 ± 4.00%) as compared to FSE (90.30 ± 1.53%). Results obtained from cell cultures tests pointed to the potential of prepared NLCs for FSE brain targeting and controlled release.
Publisher: Wiley
Relation: Journal of biomedical materials research. Part B, Applied biomaterials
Identifier: oai:repository.ukim.mk:20.500.12188/17738
Identifier: http://hdl.handle.net/20.500.12188/17738Identifier: 10.1002/jbm.b.35006
Identifier: https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbm.b.35006Identifier: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jbm.b.35006Identifier: https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbm.b.35006Identifier: 110
Identifier: 6
