Subject: Aspergillus
Subject: filamentous fungi
Subject: antifungals
Subject: resistance
Subject: susceptibility testing
Year: 2012
Type: Article
Title: In Vitro Susceptibility Testing of Aspergillus and Non-Aspergillus Filamentous Moulds to Antifungal Agents: Evaluation of Three Different Methods
Author: Gordana Mirchevska
Author: Bosshard PP
Abstract: Aim: The aim of this study was to evaluate the suitability of Sensititre YeastOne (SYO) method for susceptibility profiles’ determination of non-Aspergillus moulds and of E-test for Aspergillus spp. and non-Aspergillus moulds, in comparison with the M38-A reference broth microdilution (BMD) method. Material and Methods: A total of 33 clinical isolates of filamentous fungi were tested. Results: The agreement between E-test and BMD at ± 2 dilutions was 82.4%, 83.3% and 82.4% for amphotericin B, itraconazole and voriconazole, respectively. The agreement between SYO and BMD at ± 2 dilutions was 76.5%, 66.7% and 88.2% for amphotericin B, itraconazole and voriconazole, respectively. The majority of differing results are due to higher MICs with the reference method. In particular, SYO was unable to detect some of the potentially amphotericin B resistant strains. We found that both E-test and SYO method were reproducible and served as suitable methods for antifungal susceptibility testing of moulds. Conclusion: In conclusion, both E-test and SYO method are promising, but require further investigation to identify the optimum conditions for their use in testing of susceptibility profiles of filamentous fungi to antifungal agents.
Publisher: Institute of Immunobiology and Human Genetics, Faculty of Medicine, Skopje
Relation: Macedonian Journal of Medical Sciences
Identifier: oai:repository.ukim.mk:20.500.12188/16098
Identifier: http://hdl.handle.net/20.500.12188/16098
