Year: 1993
Type: Article
Title: Non-radioisotopic typing of human leukocyte antigen class II genes on microplates
Author: Giorda, R
Author: Lampasona, V
Author: Kocova, M
Author: Trucco, M
Abstract: We describe a new nonradioisotopic method for HLA class II molecular typing performed in 96-well plates of the same size as those used in enzyme-linked immunosorbent assays (ELISA) and radioimmunoassays (RIA). Biotinylated sequence-specific oligonucleotide (SSO) probes are bound to avidin-coated plates. Digoxigenin-labeled PCR-amplified DNA samples are hybridized, washed and detected with a peroxidase conjugated antibody assay. The method was tested by performing a partial HLA DQA1 and DQB1 typing on 69 randomly selected blood samples. The results are completely concordant with a traditional SSO-PCR typing performed on the same samples. This procedure is simple, fast and could be adapted for performance in semi-automated or automated fashion using equipment already available for ELISA and RIA assays.
Publisher: INFORMA HEALTHCARE
Relation: BioTechniques
Identifier: oai:repository.ukim.mk:20.500.12188/11559
Identifier: 0736-6205
Identifier: http://hdl.handle.net/20.500.12188/11559Identifier: 15
Identifier: 5
