Subject: Basic medicine
Year: 2021
Type: Article
Type: PeerReviewed
Title: Application of molecular biological techniques and cytogenetic methods in human biomonitoring
Author: Mileva, Dragana
Author: Velickova, Nevenka
Abstract: Human biomonitoring is defined as a field of activity that confirm some pathophysiological changes in different cells or tissue in the body (blood, urine, serum) as a result of exposure of the body to some pollutants or their’s primary or secondary products. According to the fact that every day humans are directly exposed to some physical, chemical or biological agents, molecular biological technics and methods in cytogenetics are very important in this field of study. Also, very important is the choose of different cells lines as a tool in human biomonitoring studies. Micronucleus test, comet assay and sister chromatid exchanges are cytogenetic test, accepted and confirmed of WHO, are very useful as a method that can observe the cytotoxicity and genotoxicity in the organism. With these method researchers evaluate different cytological changes, as a micronucleus, nucleoplasmatic bridges, apoptosis, changes in the chromatids etc. Beside the fact that pollutants effect on the DNA, these molecular variations can be evaluated with molecular biological technics as a PCR (polymerase chain reaction), qRT PCR (quantitative real-time polymerase chain reaction) or ELISA (Enzyme-Linked ImmunoSorbent Assay). Especially, Real time PCR is very important technics because it can analyze miRNA and genetic polymorphism in different cell lines.
Publisher:
Relation: https://eprints.ugd.edu.mk/28699/
Identifier: oai:eprints.ugd.edu.mk:28699
Identifier: https://eprints.ugd.edu.mk/28699/1/KIJ%2C%20Vol.%2048.3-1-15.pdfIdentifier: https://eprints.ugd.edu.mk/28699/2/APPLICATION%20OF%20MOLECULAR%20BIOLOGICAL%20TECHNIQUES%20AND%20CYTOGENETIC%20METHODS%20IN%20HUMAN%20BIOMONITORING.pdfIdentifier: Mileva, Dragana and Velickova, Nevenka (2021) Application of molecular biological techniques and cytogenetic methods in human biomonitoring. Knowledge – International Journal, 48 (3). pp. 571-576. ISSN 1857-923X / 2545 – 4439 (print)
