Subject: Chemical sciences
Subject: Other medical sciences
Year: 2005
Type: Article
Type: PeerReviewed
Title: HPLC determination of hydrochlorothiazide in urine after solid-phase extraction
Author: Ivanova, Violeta
Author: Zendelovska, Dragica
Author: Stefova, Marina
Abstract: A simple, rapid and precise HPLC method has been developed for the assay of hydrochlorothiazide in urine. The clean-up of the urine samples was carried out by solid-phase extraction using HLB cartridges. Extraction recovery was 94.00-100.28 %. HPLC separation was performed with isocratic elution on Hypersil BDS C18 column (100 x 4.0 mm I.D., 3 µm particle size) protected with appropriate guard column. The mobile phase was 18 % acetonitrile and 0.025 mol/L solution of KH2 PO4 , pH 4 at flow rate of 0.3 mL/min. Detection of the substances was performed at 220 nm. The calibration curves were linear in the range of 2-50 µg/mL. The developed method is validated by checking its accuracy, precision and stability. The detection limit is 2 µg/mL hydrochlorothiazide. The method is proved to be convenient for routine analysis of hydrochlorothiazide in urine.
Publisher:
Relation: https://eprints.ugd.edu.mk/316/
Identifier: oai:eprints.ugd.edu.mk:316
Identifier: https://eprints.ugd.edu.mk/316/1/Ivanova-Mac.pharm.bull-HPLC_hydrochlorothiazide%20in%20urine_SPE-pdf.pdfIdentifier: Ivanova, Violeta and Zendelovska, Dragica and Stefova, Marina (2005) HPLC determination of hydrochlorothiazide in urine after solid-phase extraction. Macedonian pharmaceutical bulletin, 51 (1,2). pp. 23-28. ISSN 1409 - 8695
