Subject: Chemical sciences
Year: 2008
Type: Article
Type: PeerReviewed
Title: HPLC method for determination of verapamil in human plasma after solid-phase extraction
Author: Ivanova, Violeta
Author: Zendelovska, Dragica
Author: Stefova, Marina
Author: Stafilov, Trajče
Abstract: A simple, rapid and precise HPLC method has been developed for the assay of verapamil in human plasma. The clean up of the plasma samples was tested using several adsorbents for solid-phase extraction and best recovery was obtained using mixed-mode cartridges (HLB - hydrophilic-lipophilic balance) ranging between 94.70 and 103.71%. HPLC separation was performed with isocratic elution on Lichrospher 60 RP-select B column (250 mm × 4mmI.D., 5 μmparticle size). The mobile phasewas 40%acetonitrile and 0.025mol/LKH2PO4 with pH 2.5 at flow rate of 1 mL/min.Diltiazemwas used as internal standard and the detection wavelength was 200 nm. The calibration curves were linear in the range of 10–500 ng/mL. The developed method is convenient for routine analysis of verapamil in human plasma.
Publisher: Elsevier
Relation: https://eprints.ugd.edu.mk/313/
Identifier: oai:eprints.ugd.edu.mk:313
Identifier: https://eprints.ugd.edu.mk/313/1/Ivanova-JBBM-verapamil%2070%20%282008%29%201297-1303.pdfIdentifier: Ivanova, Violeta and Zendelovska, Dragica and Stefova, Marina and Stafilov, Trajče (2008) HPLC method for determination of verapamil in human plasma after solid-phase extraction. Journal of Biochemical and Biophysical Methods, 70. pp. 1297-1303.
