Subject: Basic medicine
Year: 2004
Type: Article
Type: PeerReviewed
Title: A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans
Author: Mitreva, Makedonka
Author: Elling, Axel A.
Author: Dante, Mike
Author: Kloek, Andrew P
Author: Kalyanaraman, A.
Author: Aluru, S.
Author: Clifton, Sandra W.
Author: Bird, David M.
Author: Baum, Thomas J.
Author: McCarter, James P.
Abstract: Plant-parasitic nematodes are important and cosmopolitan pathogens of crops. Here, we describe the generation and analysis of 1928 expressed sequence tags (ESTs) of a splice-leader 1 (SL1) library from mixed life stages of the root-lesion nematode Pratylenchus pene- trans. The ESTs were grouped into 420 clusters and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. Approxi- mately 80% of all translated clusters show homology to Caenorhabditis elegans proteins, and 37% of the C. ele- gans gene homologs had confirmed phenotypes as as- sessed by RNA interference tests. Use of an SL1-PCR approach, while ensuring the cloning of the 5¢ ends of mRNAs, has demonstrated bias toward short tran- scripts. Putative nematode-specific and Pratylenchus - specific genes were identified, and their implications for nematode control strategies are discussed.
Publisher:
Relation: https://eprints.ugd.edu.mk/2567/
Identifier: oai:eprints.ugd.edu.mk:2567
Identifier: https://eprints.ugd.edu.mk/2567/1/A%20survey.pdfIdentifier: Mitreva, Makedonka and Elling, Axel A. and Dante, Mike and Kloek, Andrew P and Kalyanaraman, A. and Aluru, S. and Clifton, Sandra W. and Bird, David M. and Baum, Thomas J. and McCarter, James P. (2004) A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans. Mol Gen Genomics, 272 (2). pp. 138-148.
