Subject: Other medical sciences
Year: 2005
Type: Article
Type: PeerReviewed
Title: Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii
Author: Mitreva, Makedonka
Author: Bird, David M.
Author: Cheysen, Godelieve
Abstract: The beet cyst nematode Heterodera schachtii is an important pathogen worldwide, but its molecular characterization has been limited to studying individual genes of interest. We undertook a high-throughput genomic approach and drastically increased the number of available sequences for this parasite. A total of 2,662 expressed sequence tags were grouped into 1,212 clusters representing a nonredundant catalog of H. schachtii genes. Im- plementing a bioinformatic workflow, we identified 50 sequences coding for candidate secreted proteins. All of these contain a putative signal peptide required for entry into the secretory pathway and lack any transmembrane domain. Included are previously postulated cell-wall- degrading enzymes and other parasitism-related genes. Moreover, we provide the first report of an arabinogalactan endo-1,4-β-galactosidase enzyme (EC 3.2.1.89) in ani- mals. As sequence data increase at a rapid rate, developinghigh-throughput genomic screening is a necessity. The in silico approach described here is an effective way to identify putative secreted proteins and prioritize candidates for further studies.
Publisher: Published online
Relation: https://eprints.ugd.edu.mk/2488/
Identifier: oai:eprints.ugd.edu.mk:2488
Identifier: https://eprints.ugd.edu.mk/2488/1/detection_19%2Ca.22.pdfIdentifier: Mitreva, Makedonka and Bird, David M. and Cheysen, Godelieve (2005) Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii. Parasitol Res , 98. pp. 414-424.
