Subject: Basic medicine
Subject: Biological sciences
Subject: Chemical sciences
Subject: Physical sciences
Year: 2005
Type: Article
Type: PeerReviewed
Title: Enzymatic formation of ions and their detection at a three-phase electrode
Author: Gulaboski, Rubin
Author: Pereira, Carlos M.
Author: Cordeiro, M Natália D S
Author: Bogeski, Ivan
Author: Silva, A. Fernando
Abstract: An electrochemical method for the detection of enzymatically created anions is described that uses a thin-film electrode with decamethylferrocene as an electroactive redox probe. The enzymatic oxidation of glucose with enzyme glucose oxidase produces gluconic acid as a final product. The oxidation of decamethylferrocene dissolved in the thin-nitrobenzene film, that is spread on the working graphite electrode and submerged in the aqueous solution containing glucose and glucose oxidase, is followed by the up-take of gluconate anions from the aqueous phase to nitrobenzene. The peak currents of the square-wave voltammetric responses of that system are a linear function of the glucose concentration in the milimolar range from 0.1 mmol/L to 0.7 mmol/L (R2=0.994).
Publisher:
Relation: https://eprints.ugd.edu.mk/155/
Identifier: oai:eprints.ugd.edu.mk:155
Identifier: https://eprints.ugd.edu.mk/155/1/__ugd.edu.mk_private_UserFiles_rubin.gulaboski_Desktop_GULABOSKI-MY%20pdf%20PUBLICATIONS_JOSEC%202005-Enzymatic%20creation%20of%20anions%20at%203%20phase%20electrode.pdfIdentifier: Gulaboski, Rubin and Pereira, Carlos M. and Cordeiro, M Natália D S and Bogeski, Ivan and Silva, A. Fernando (2005) Enzymatic formation of ions and their detection at a three-phase electrode. Journal of Solid State Electrochemistry, 9 (6). pp. 469-474. ISSN 1432-8488
Identifier: doi:10.1007/s10008-005-0696-0
